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Fig. 5 | BMC Microbiology

Fig. 5

From: The ATP-dependent RNA helicase HrpB plays an important role in motility and biofilm formation in Xanthomonas citri subsp. citri

Fig. 5

Pathogenicity assay and growth of X. citri subsp. citri strains in planta. a Symptoms were analyzed on the lower the surfaces of sweet orange leaves at 21 days post-inoculation (d.p.i.). b In vivo bacterial growth in populations of wild-type, ∆hrpB and a complementary strain of Xanthomonas citri subsp. citri (X. citri) that were inoculated on the sweet orange leaves by spraying the leaves with bacteria at a concentration of 108 CFU/mL. Bacterial cells were extracted from the leaves at different time points after inoculation. The leaves were homogenized in MgCl2 and then plated on appropriate media after serial dilution. Colonies were counted after a 2-day incubation at 28 °C. Abbreviations: wt-p53, wild-type strain 306 with empty vector pUFR053; ∆hrpB, hrpB mutant; and ∆hrpB-p53hrpB, complemented hrpB mutant. Values are expressed as the means ± standard deviations of three independent experiments

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