Table 2 Oligonucleotides used for mutant and complement strains construction in this work
Primer | Sequence |
|---|---|
Amplification of the Cm cassette | |
Forward | GAATTTTTTCGCTATAGTGTAGGCTGGAGCTGCTTC |
Reverse | GAATTTTTTCGCTATACATATGAATATCCTCCTTAG |
Mutagenesis | Â |
Forward | TATTCTTTAGGGCTATGGTTTTTCATTTTTTACCGGAAGTTACCGACGTTGTGTAGGCTGGAGCTGCTTC |
Reverse | AGTCTCGCGTGGAAGCTGCGGTATGGGTGCATCAGGAGCGCATTTTCTGACATATGAATATCCTCCTTAG |
Mutagenesis confirmation | |
Forward | TACCGGAAGTTACCGACGTT |
Reverse | ATCAGGAGCGCATTTTCTGA |
Genetic Complementation | |
Forward | GCTATAGTCGACGCGAGAAAATACGACAAAAG |
Reverse | GTAAGGATCCCACATGCTGAAGAAAATGAA |