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Table 1 Strains and plasmids used in this work

From: The virulence factor ychO has a pleiotropic action in an Avian Pathogenic Escherichia coli (APEC) strain

Strain or plasmid Characteristics Reference or source
SEPT362 APEC strain isolated from a septicemic laying hen [24]
SEPT362Cm SEPT362 with plasmid pKD46Cm This study
ychO ychO mutant This study
C.∆ychO ychO mutant complemented with plasmid pC.∆ychO This study
pACYC184 cloning vector New England Biolabs
pKD4 pANTS derivative plasmid containing FRT-flanked kanamycin resistance [63]
pKD3 pANTS derivative plasmid containing FRT-flanked chloramphenicol resistance [63]
pKD46 λ Red recombinase expression plasmid [63]
pKD46Cm Plasmid pKD46 with a chloramphenicol cassette insertion This study
pC.∆ychO Plasmid pACYC184 with ychO This study