Table 1 Strains and plasmids used in this work
Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
SEPT362 | APEC strain isolated from a septicemic laying hen | [24] |
SEPT362Cm | SEPT362 with plasmid pKD46Cm | This study |
∆ychO | ychO mutant | This study |
C.∆ychO | ychO mutant complemented with plasmid pC.∆ychO | This study |
pACYC184 | cloning vector | New England Biolabs |
pKD4 | pANTS derivative plasmid containing FRT-flanked kanamycin resistance | [63] |
pKD3 | pANTS derivative plasmid containing FRT-flanked chloramphenicol resistance | [63] |
pKD46 | λ Red recombinase expression plasmid | [63] |
pKD46Cm | Plasmid pKD46 with a chloramphenicol cassette insertion | This study |
pC.∆ychO | Plasmid pACYC184 with ychO | This study |