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Table 2 Summary of Phage related regions identified by PHAST in the 5 genomes. Phage sequences identified in this study

From: Comparative genomic analysis of toxin-negative strains of Clostridium difficile from humans and animals with symptoms of gastrointestinal disease

PHAST region identifier

Length of prophage

PHAST score

No of predicted CDS

Relative position on genome

Predicted Phage

GC content

Location on Genomic scaffolds

C difficile P29 genome

 Region_4

97.4Kb

150

126

1732045-1829500

PHAGE_Clostr_CDMH1_NC_024144

28.7

27.1, 30.1, 40.1, 34.1, 36,1, 5.1, 47.1 and 16.1

 Region_8

113.6Kb

150

101

3773336-3886941

PHAGE_Geobac_virus_E2_NC_009552

40.8

5.1, 19.1 and 22.1

 Region_9

52.8Kb

140

65

4233532-4286418

PHAGE_Clostr_phiCD6356_NC_015262

29.9

8.1, 26.1, 41.1 and 42.1

 Region_10

21.3Kb

100

22

4290994-4312349

PHAGE_Clostr_phiC2_NC_009231

36.4

In over 20 very small scaffolds

C. difficile H3 genome

 Region_2

41.1Kb

150

63

925289-966449

PHAGE_Clostr_phiCD6356_NC_015262

28.5

22.1 and 31.1

 Region_3

50.1Kb

110

66

1114600-1164714

PHAGE_Clostr_phiC2_NC_009231

28.1

18.1 and 5.1

 Region_6

31.5Kb

110

47

4070500-4102080

PHAGE_Clostr_phiC2_NC_009231

29.4

26.1, 27.1, 28.1, 32.1, 33.1

C. difficile 5.3 genome

 Region_3

57.9Kb

140

87

1555612-1613526

PHAGE_Clostr_phiC2_NC_009231

28.6

1.1

 Region_4

45.1Kb

150

49

1741883-1787019

PHAGE_Clostr_phiSM101_NC_008265

27.2

1.1

C.difficle 19.3 genome

 Region_3

56.8Kb

140

74

1700180-1757059

PHAGE_Clostr_phiC2_NC_009231

28.8

1.1

C. difficile 22.1 genome:

 Region_3

56.8Kb

140

74

1703858-1760737

PHAGE_Clostr_phiC2_NC_009231

28.8

3.1