Fig. 7

Generation of intracellular ROS by chromate. a Cytoplasmic ROS content was evaluated measuring the H2DCFDA probe activation in cells of, wild strain O. tritici 5bvl1 (black bar); chrC mutant (grey bar); chrF mutant (white bar) and chrC/chrF mutant (dashed bar), treated with indicated concentrations of chromate. b Comparative fluorescence signals of cells untreated (solid line) or treated (dashed line) with 1 mM chromate over time for wild strain O. tritici 5bvl1 (♦); chrC mutant (▲); chrF mutant (●) and chrC/chrF mutant (x)