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Fig. 3 | BMC Microbiology

Fig. 3

From: Two superoxide dismutases from TnOtchr are involved in detoxification of reactive oxygen species induced by chromate

Fig. 3

Determination of the molecular mass of ChrC and ChrF by gel filtration. Chromatography was performed on Superdex 200 10/300 GL packed column at a flow rate of 0.5 mL/min with 50 mM Tris–HCl, 0.15 M NaCl, pH 7.4. Kav values were calculated using the equation: Kav = (Ve-Vo)/(Vc-Vo), where Vo is column void volume, Ve is elution volume, and Vc is geometric column volume. The studied proteins are represented by open symbols: ChrC (); ChrF (□)

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