Fig. 3From: Two superoxide dismutases from TnOtchr are involved in detoxification of reactive oxygen species induced by chromateDetermination of the molecular mass of ChrC and ChrF by gel filtration. Chromatography was performed on Superdex 200 10/300 GL packed column at a flow rate of 0.5 mL/min with 50 mM Tris–HCl, 0.15 M NaCl, pH 7.4. Kav values were calculated using the equation: Kav = (Ve-Vo)/(Vc-Vo), where Vo is column void volume, Ve is elution volume, and Vc is geometric column volume. The studied proteins are represented by open symbols: ChrC (○); ChrF (□)Back to article page