Table 3 Strains and plasmids used in the study

ST4/74

S.Typhimurium strain ST4/74. Reference strain.

[25]

LJ584

S.Typhimurium strain ST4/74, WITS1, kan^R

This study

LJ585

S.Typhimurium strain ST4/74, WITS2, kan^R

This study

LJ586

S.Typhimurium strain ST4/74, WITS3, kan^R

This study

LJ587

S.Typhimurium strain ST4/74, WITS4, kan^R

This study

LJ588

S.Typhimurium strain ST4/74, WITS5, kan^R

This study

LJ589

S.Typhimurium strain ST4/74, WITS6, kan^R

This study

LJ590

S.Typhimurium strain ST4/74, WITS7, kan^R

This study

LJ591

S.Typhimurium strain ST4/74, WITS8, kan^R

This study

LJ684

S.Typhimurium strain ST4/74, ΔkdgR, kan^R

This study

LJ678

S.Typhimurium strain ST4/74, ΔptsN, kan^R

This study

Top10

E. coli cloning strain

Invitrogen

Plasmids

pCR®2.1-TOPO

TOPO-cloning vector, amp^R, kan^R

Invitrogen

pACYC177

Cloning vector, amp^R, kan^R

[32]

pKD46

Plasmid with λ-Red recombinase expressed from arabinose inducible promoter

[29]

pKD4

Template plasmid for λ-Red mutagenesis, kan^R

[29]