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Fig. 5 | BMC Microbiology

Fig. 5

From: Salmonella Typhimurium undergoes distinct genetic adaption during chronic infections of mice

Fig. 5

The kdgR-SNP elicits constitutive repression of its target gene kdgK. The wt, the kdgR-SNP and a ΔkdgR strain were grown in M9 minimal media at 37 °C with shaking until mid-logarithmic phase. At this point a sample from each strain was collected and the KdgR inducer KDG was added to all cultures. Cultures were re-incubated for an hour and a sample from each culture was collected. Total RNA was extracted from all samples, and the level of the kdgK transcript was assessed by northern blot analysis using a radioactive probe specific for kdgK

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