Table 2 Primers used in this study. Underlined sequences are complementary sequences to adjacent segments
Oligonucleotide | Sequence |
|---|---|
5’srfA-A-∆Leu-up-F | 5’-CAAGATACGTATCCT-3’ |
3’srfA-A-∆Leu–SOE-up-R | 5’-CAGCATTCCCTCCTGAGTCGGAAGCGTCAG-3’ |
5’srfA-A-∆Leu–SOE-down-F | 5’-CTGACGCTTCCGACTCAGGAGGGAATGCTG-3’ |
3’srfA-A-∆Leu-down-R | 5’-CCACTTGATGTAATC-3’ |
5’srfA-B-∆Asp -up-F | 5’-CAGCATTATCCTGTATC-3’ |
3’srfA-B-∆Asp–SOE-up-F | 5’-AGCAGACGCCTCCATTGGCCGCTCGAAATC-3’ |
5’srfA-B-∆Asp–SOE-down-F | 5’-GATTTCGAGCGGCCAATGGAGGCGTCTGCT-3’ |
3’srfA-B-∆Asp-down-R | 5’-TTGCCAAACGGCG-3’ |
5’srfA-B-∆Leu-up-F | 5’-ATGGAGGCGTCTGCT-3’ |
3’srfA-B-∆Leu–SOE-up-F | 5’-GCTAAATTGACTCATTTGCCAAACGGCGAA-3’ |
5’srfA-B-∆Leu–SOE-down-R | 5’-TTCGCCGTTTGGCAAATGAGTCAATTTAGC-3’ |
3’srfA-B-∆Leu-down-R | 5’-CGGCTTTTGTTCGCG-3’ |
5’pKS-1058-ERM-F | 5’-CTTTGGCGTGTTTCATTGCTTG-3’ |
3’pKS-1058-ERM-R | 5’-GGTTCGTGTTCGTGCTGACTTG-3’ |