Strain/plasmid | Relevant genotype/description | Reference |
---|---|---|
E. coli | Â | Â |
DH5α | recA1, endA1, lacZDM15 | New England Biolabs |
JM110 | F',traD36proA+B+lacIqlacZΔM15/damdcmsupE44hsdR17 thi leu thr rpsL lacY galK galT ara tonA tsx∆ (lac-proAB) | Transgen Biolabs |
B. subtilis | Â | Â |
B. subtilis PB2 | B. subtilis 168 trpC2 | Chester Price’ lab (UCDavis, USA) |
B. subtilis PB2-L1 | Derivative of B. subtilis PB2 Produces surfactin | Our labs |
B. subtilis LS1 | Lacking the third D-leucine module from B. subtilis PB2-L1 | This work |
B. subtilis LS6 | Lacking the fifth L-aspartate module from B. subtilis PB2-L1 | This work |
B. subtilis LS9 | Lacking the sixth D-leucine module from B. subtilis PB2-L1 | This work |
Plasmids | Â | Â |
pMD19T-simple | TA cloning vector; AmpR | TAKARA |
pKS2 | Thermosensitive vector; KanR,ErmR | Our labs |
pKS2-srfA-C-∆Leu | Third D-leucine module knock-out vector; KanR,ErmR | This work |
pKS2-srfA-B-∆Asp | Fifth L-aspartate module knock-out vector; KanR,ErmR | This work |
pKS2-srfA-B-∆Leu | Sixth D-leucine module knock-out vector; KanR,ErmR | This work |