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Table 1 Bacterial strains and plasmids used in this study

From: Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity against Fusarium moniliforme

Strain/plasmid

Relevant genotype/description

Reference

E. coli

  

DH5α

recA1, endA1, lacZDM15

New England Biolabs

JM110

F',traD36proA+B+lacIqlacZΔM15/damdcmsupE44hsdR17 thi leu thr rpsL lacY galK galT ara tonA tsx∆ (lac-proAB)

Transgen Biolabs

B. subtilis

  

B. subtilis PB2

B. subtilis 168 trpC2

Chester Price’ lab (UCDavis, USA)

B. subtilis PB2-L1

Derivative of B. subtilis PB2 Produces surfactin

Our labs

B. subtilis LS1

Lacking the third D-leucine module from B. subtilis PB2-L1

This work

B. subtilis LS6

Lacking the fifth L-aspartate module from B. subtilis PB2-L1

This work

B. subtilis LS9

Lacking the sixth D-leucine module from B. subtilis PB2-L1

This work

Plasmids

  

pMD19T-simple

TA cloning vector; AmpR

TAKARA

pKS2

Thermosensitive vector; KanR,ErmR

Our labs

pKS2-srfA-C-∆Leu

Third D-leucine module knock-out vector; KanR,ErmR

This work

pKS2-srfA-B-∆Asp

Fifth L-aspartate module knock-out vector; KanR,ErmR

This work

pKS2-srfA-B-∆Leu

Sixth D-leucine module knock-out vector; KanR,ErmR

This work