Fig. 2From: Identification of novel surfactin derivatives from NRPS modification of Bacillus subtilis and its antifungal activity against Fusarium moniliforme Knockout strategy of marker-free gene. Note that recombination may occur, both at the upstream fragment of the target gene, as shown below, and at the downstream fragment. In both cases, the final connection result of the chromosome is the same thing. All of intermediation process was identified by polymerase chain reactionBack to article page