Table 2 List of strains used in this study

EB304

MG1655, Δcas3::apra

[44]

BSN22

W3110, Δhns::cat

[46]

BW25113

F⁻ rrnB ΔlacZ4748 (::rrnB-3) hsdR514 Δ (araBAD) 567 Δ (rhaBAD) 568 rph-1 λ⁻

[47]

Bacterial strains related to BW25113

BW39121

+ Δhns::kan

[38]

JW0462

+ ΔhtpG::kan

[41]

BW40114

+F’ (proAB ⁺ lacI ^qZΔM15::Tn10) lacUV5-cas3 cat::araBp8-casA

[27]

BW39651

+ λT3 spacer

[38]

BW39671

+ λT3 Δhns::kan

[38]

BW39183

+ Δcas1::kan

[38]

IIB848

+ λT3 Δcas3::apra

recombineering using pKD46

IIB870

+ λT3 Δcas3::apra

P1. IIB848 × BW39651

IIB965

+ λT3 Δcas1::kan

P1. BW39183 × BW39651

IIB966

+ λT3 Δcas1::kan Δhns::cat

P1. BSN22 × IIB965

IIB969

+ λT3 lacUV5-cas3 cat::araBp8-casA

P1. BW40114 × BW39651

IIB969e

+ λc + λT3 lacUV5-cas3 cat::araBp8-casA

Selection of λ^r colony with phage acquired spacer

IIB1039

+ λc + λT3 Δcas1::kan

P1. BW39183 × IIB969e (selection Km^r Chl^s and PCR of the CRISPR-1 region)

IIB1040

+ λc + λT3 Δcas1::kan Δhns::cat

P1. BSN22 × IIB1039

IIB1043

+λc + λT3 Δcas1::kan ^S

Removal of kan cassette by pCP20 plasmid

IIB1063

+λc + λT3 lacUV5-cas3 cat::araBp8-casA ΔhtpG::kan

IIB1065

+λc + λT3 Δcas1::kan ^SΔhtpG::kan

P1. JW0462 × IIB1043

IIB1066

+λc + λT3 Δcas1::kan ^SΔhtpG::kan Δhns::cat

P1. BSN22 × IIB1065