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Table 1 E. coli cells lacking H-NS show temperature-dependent resistance to phage

From: Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

strain genotype Plaque forming units (PFUs)
30 °C 37 °C
BW25113 hns + 3.80 × 1010 ± 7 × 109 4.56 × 1010 ± 9 × 109
BW39121 Δhns 1.20 × 1010 ± 1.8 × 109 4.23 × 1010 ± 6.6 × 1010
BW39651 hns + + λT3 3.66 × 1010 ± 7.57 × 109 4.03 × 1010 ± 1.2 × 1010
BW39671 Δhns + λT3 ~4 × 105 4.16 × 1010 ± 5.5 × 109
  1. λvir
  2. Cell lawns were infected with phage dilutions (from 10−2 to 10−8) and incubated at either 30 °C or 37 °C. Cells lacking H-NS (Δhns) or containing H-NS (hns +) had fully operational CRISPR-Cas systems that were engineered with an anti-λ spacer (λT3) as indicated. The average of at least three independent experiments are shown