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Fig. 1 | BMC Microbiology

Fig. 1

From: Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

Fig. 1

Cas3 is transcribed in cells lacking H-NS at both temperatures of incubation in stationary phase of growth. qPCR analysis extended to Δhns Δcas1 and hns + Δcas1 cells containing λT3 and/or λc anti-lambda spacer. Relative expression levels of cas3 transcripts are measured in cells grown to stationary phase at indicated temperatures of incubation with groES as reference gene. Error bars represent normalized error of respective duplicates. Histogram bars marked with different letters (a for hns + samples, and b, bc, cd and d for Δhns samples) indicate significant difference between expression values, while samples that share a letter in the notation (e.g. b, bc, cd or d and cd) do not have statistically different expression values. Cas3 expression levels were compared across all samples, and two expression values were considered significantly different as evaluated by One-way ANOVA Duncan Multiple Range post-hoc test (p < 0.05)

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