Fig. 5

Extracellular proteins analysis by SDS-PAGE. Proteins samples were obtained from yeast cultures of Tetracladium sp. (a and b) and M. gelida (c) in YM medium supplemented with glucose (a and c) or soluble starch (b). Samples were fractioned with ammonium sulfate. The 60 % fraction, which had the highest amylase activity (a and b), and the 70 and 80 % fractions (c) that had the highest cellulase activity are shown. Serial dilutions of the samples were made (indicated at the top of gels) and the degree of the determined amylase or cellulase activity by the well assay is indicated at the bottom of each gel lane as follows: highest (+++) and lowest (+) recorded activity. The arrows indicate the protein band associated to amylase (a and b) or cellulase activity (c). M, protein marker