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Fig. 2 | BMC Microbiology

Fig. 2

From: Emergence of nontoxic mutants as revealed by single filament analysis in bloom-forming cyanobacteria of the genus Planktothrix

Fig. 2

PCR amplification of the entire mcy gene cluster from single Planktothrix rubescens filaments to detect mutations by PCR size polymorphism. a Amplification of DNA fragments (approx. 3 kb) of the entire mcy gene cluster from DNA isolated from one individual filament (No 11, Mondsee, 15 Mar 2012). The nucleotide pos. represents the binding position of the forward primer according to the sequence of the mcy gene cluster from P. agardhii NIVA-CYA126/8 (AJ441056), (Additional file 6). b Amplification of DNA fragment using primer pair Fmcy2+/− from individual filaments No 59–76 isolated from Zürichsee (20 Jun 2012). Filament No 66 shows a deletion in mcyD. c Amplification of DNA fragment using primer pair Fmcy8+/Fmcym8- (1.75 kb) from individual filaments No 88–100 isolated from Wörthersee (3 Apr 2012). The larger PCR products (Filament No 88, 94, 96, 100) indicate the insertion into the IGS region (1423 bp) between mcyE and mcyG. M, PstI lambda DNA size marker. Positive control (+) was amplified from P. agardhii NIVA-CYA126/8 (AJ441056)

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