Fig. 5

Distribution of the centers of the digestion fragments from the nucleoid sample relative to TSS (a) and TES (b). Positions of the fragment centers were estimated with the help of tag-cross correlation analysis (see Methods for details). Green arrows indicate direction of transcription. Positions of 5’-ends of the tags on the positive and negative strands were shifted by +/−25 bp respectively (the half-distance at which the maximum in cross-correlation profile is observed). The tag frequency profiles were smoothed by averaging in 50-bp running window. c, d GC-content of the MNase resistant fragments at TSS (c) and TES (d). GC-content is averaged for each position in the TSS and TES proximal regions over all annotated E. coli genes. Unsmoothed and smoothed profiles are shown with thin grey lines and thick green lines respectively