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Table 1 Primers and probes

From: A prospective study of two isothermal amplification assays compared with real-time PCR, CCNA and toxigenic culture for the diagnosis of Clostridium difficile infection

Gene

Primers/Probes

PCR-product (bp)

Acc-number

Gene region (bp)

tcdA

tcdA F: 5-´ATTCCAATACAAGYCCTGTAGAAAA-3´

85

AM180355

796102–796186

tcdA R: 5- TTTATGTATTCAAGARCAATATCACTGACT-3´

tcdA-S: 5´Fam-RATTTACATTTTGTATGGATAGGTGGAG-BHQ-1-3´

tcdB

tcdBF: 5´-AACAGGTGTATTTAGTACAGAAGATGGATT-3´

85

AM180355

793077–793161

tcdBR: 5´-AATTGCTTCTCCTTCTAGGTTTTCAT-3´

tcdB-S: 5´-Hex-AAATATTTTGCCCCAGCTAATACACTT-BHQ-1-3´

tcdB027a

tcdBF: 5´-AACAGGTGTATTTAGTACAGAAGATGGATT-3´

85

FN665654

708928–709012

027tcdBR*: 5´-AATTGCTTCCCCCTCTAGATTTTCAT-3´

027tcdB-S:5´-Hex-AAATATTTTGCTCCAGCAGATACACTT-BHQ-1-3´

16S

CdF: 5´-TGTACACACGGATAACATACCGAAA-3´

131

AM180355

125189–125289

CdR:5´-CCGTTACCTTACCAACTAGCTAATCA-3´

CdS5´-Fam-CATCTCTTGAATATCAAAGGTGAGCCAGTACAGG-BHQ-1-3´

cdtB

cdtBfor: 5´GATGATCCATTTATCCCAAATAACAA-3´

132

FN665654

2833044–2833175

cdtBrev:5´GTCCTTAATAGTATATCCATTTCGTTCATATG-3´

cdtBS:5´Hex-TTCTTTGACCCAAAGTTGATGTCTGATTGGG-BHQ-1-3´

slpA (Kato et al. 2004)

slpAcom22 : 5'-GCWGTYTCTATTCTATCDTYWCC-3'

23

AM180355

3253526–3254737

slpAcom19 : 5'-GTTGGGAGGAATTTAAGRAAtG-3'

22

  1. aBolded bases represent a difference from the standard tcdB primer or probe