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Fig. 2 | BMC Microbiology

Fig. 2

From: A multi-drug resistant Salmonella Typhimurium ST213 human-invasive strain (33676) containing the bla CMY-2 gene on an IncF plasmid is attenuated for virulence in BALB/c mice

Fig. 2

Strain 33676 has functional SPI-1 and SPI-2-encoded T3SSs and secretes FljB flagellin. Analysis of secreted proteins from culture supernatants was performed for strains SL1344, 33676 and ΔSPI-1 or ΔSPI-2 grown in LB (a) or N-MM (b). TCA-precipitated proteins SipA, SipB, SipC and SipD, secreted through the T3SS encoded in SPI-1 (a), and protein SseB, secreted through the T3SS encoded in SPI-2 (b), were detected by Coomassie blue staining and by Western blot, respectively. FliC and FljB (a) are flagellin subunits proteins whose secretion is independent of SPI-1

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BMC Microbiology

ISSN: 1471-2180

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