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Table 3 List of genes silenced by in planta RNAi method in pant parasitic nematodes

From: Identification and functional analysis of secreted effectors from phytoparasitic nematodes

Gene name/genbank accession no.

Putative functions of target genes

Nematode species

Observed Phenotype

References

In planta RNAi

AW871671

Integrase

M. incognita

>90 % reduction in established nematodes

[96]

AW828516

Splicing factor

M. incognita

>90 % reduction in established nematodes

[96]

16D10 (DQ841121-DQ841123)

Secreted peptide

M. arenaria,

63 %-90 % reduction no. of galls and gall size

[89]

M. incognita,

 

[115]

M. javanica, M. hapla,

  

M. arenaria

  

MSP

Major sperm protein

H. glycines

Up to 68 % reduction in nematode eggs

[95]

MjTIS-11

Putative transcription factor

M. javanica

 

[97]

Hg-rps-3a, CB379877

Ribosomal protein 3a

H. glycines

87 % reduction in female cysts

[98]

Hg-rps-4, CB278739

Ribosomal protein 4

H. glycines

81 % reduction in female cysts

[98]

Hg-spk-1, BI451523.1

Spliceosomal SR protein

H. glycines

88 % reduction in female cysts

[98]

Hg-snb-1, BF014436

Synaptobrevin

H. glycines

93 % reduction in female cysts

[98]

4G06, AF469060

Ubiquitin-like

H. schachtii

23 %-64 % reduction in developing females

[94]

3B05, AF469058

Cellulose binding protein

H. schachtii

12 %-47 % reduction in developing females

[94]

8H07, AF500024

SKP1-like

H. schachtii

>50 % reduction in developing females

[94]

10A06, AF502391

Zinc finger protein

H. schachtii

42 % reduction in developing females

[94]

Y25, CB824330

Beta subunit of the COPI complex

H. glycines

81 % reduction in nematode eggs

[99, 116]

Prp-17, AF113915

Pre-mRNA splicing factor

H. glycines

79 % reduction in nematode eggs

[99]

Mispc3, Miduox

 

M. incognita

Reduction of nematode number root, retarded female development

[117]

Cpn-1 , GU074018

Unknown protein

H. glycines

95 % reduction in nematode eggs

[99]

Tyrosine Phosphatase, Mitochondrial stress-70 protein precursors, Lactate dehydrogenase

 

M. incognita

Reduced no. of established females

[100]

Mi-Rpn7

 

M. incognita

Reduction in reproduction and motility

[118]

Parasitism gene 8D05

 

M. incognita

Reduction in gall number

[110]

Calreticulin-Mi-CRTN

 

M. incognita

Reduction in gall number

[119]

Fatty acid and retinol binding protein (Mj-far-1 )

 

M. javanica

Ceased development of nematodes along with reduction in giant cell number

[102]

FMRFamide-likepeptides (flp-14,flp-18)

 

M. incognita

Reduction in gall number, fecundity, female development and increased root growth of transgenics

[101]

Pv010

 

P. vulnus

Reduced nematode multiplication with no visible lesions

[120]

Effector gene, Mc16D10L

 

M. chitwoodi

Reduction in fecundity and pathogenicty

[90]

Effector gene, Gp-hyp

 

G. pallida

Reduction in nematode parasitism

[121]