Mutations in the nucleotide binding and hydrolysis domains of Helicobacter pylori MutS2 lead to altered biochemical activities and inactivation of its in vivo function

Table 4 Recombination frequencies determined for H. pylori strains

Strain genotype	n^a	Recombinant frequency (x10⁻⁴)	Relative value	P value (MWU)
Wt	30	4.96 (±3.78)	1
Wt + hpmutS2	14	0.963 (±0.97)	0.19	<0.0001
Wt + hpmutS2-G338R	15	4.18 (±4.87)	0.84	0.802
Wt + hpmutS2-E413A	18	23.4 (±17.8)	4.71	<0.0001
hpmutS2 ⁻	36	39.2 (±29.2)	7.9	<0.0001
hpmutS2 ⁻ + hpmutS2	27	1.00 (±4.29)	0.20	0.0003
hpmutS2 ⁻ + hpmutS2-G338R	30	25.2 (±22.9)	5.08	<0.0001
hpmutS2 ⁻ + hpmutS2-E413A	21	45.2 (±54.4)	9.11	<0.0001

The recombination frequencies were calculated as the number of streptomycin resistance colonies per recipient cfu. Values correspond to the average and standard deviation. P values were calculated using the Mann–Whitney U test. n^a No. of independent determinants

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