| |
No nucleotide
|
+ ATP
|
+ ADP
|
+ ATPγS
|
|---|
|
Single-stranded DNA
|
|
HpMutS2
|
24.82 ± 1.15
|
32.08 ± 5.32
|
28.11 ± 5.15
|
28.5 ± 3.68
|
|
HpMutS2-G338R
|
40.19 ± 8.73
|
40.45 ± 9.52
|
38.87 ± 8.79
|
ND
|
|
HpMutS2-E413A
|
6.50 ± 0.55
|
7.23 ± 0.65
|
6.90 ± 1.4
|
ND
|
|
HpMutS2ΔSmr-D30A
|
2.12 ± 1.67
|
ND
|
ND
|
ND
|
|
Holliday junction
|
|
HpMutS2
|
26.49 ± 3.95
|
37.77 ± 8.59
|
18.85 ± 1.9
|
17.44 ± 0.84
|
|
HpMutS2-G338R
|
41.59 ± 9.53
|
43.7 ± 11.42
|
41.6 ± 10.27
|
ND
|
|
HpMutS2-E413A
|
4.92 ± 1.25
|
1.27 ± 1.01
|
6.77 ± 3.53
|
ND
|
|
HpMutS2ΔSmr-D30A
|
3.34 ± 1.67
|
ND
|
ND
|
ND
|
- Reaction rates were estimated by dividing the total product formed by corresponding incubation time obtained from the linear range of enzymatic activity (Figs. 6a-f). The error values represent standard deviation from two or more different time points. ND, not determined
