Fig. 2

Nucleotide binding affinities of a HpMutS2 b HpMutS2-G338R and c HpMutS2-E413A. Tryptophan fluorescence assays described in the Methods section were used to determine the nucleotide binding affinities of proteins. The dissociation constants were calculated by non-linear regression analysis of plots of ΔF/ΔFmax versus the ligand concentration. ΔF: magnitude of the difference between the observed fluorescence intensity at a given ligand concentration and the fluorescence intensity in the absence of any ligand, ΔFmax: difference at an infinite ligand concentration