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Table 1 Specificity test of qPCR markers. The marker for each isolate is, at least, specific to the other two isolates used in this study

From: Independent mitochondrial and nuclear exchanges arising in Rhizophagus irregularis crossed-isolates support the presence of a mitochondrial segregation mechanism

   R. irregularis DAOM G. cerebriforme DAOM227022 Water
  Marker\DNA 197198 240415 234328 197198/240415 240415/234328 197198/234328 242422 234179
Singleplex 197198 X (19) - - X (19) - X (19) X (19) - - -
240415 - X (21) - X (21) X (21) - - - - -
234328 -   X (26) - X (26) X (27) - X (27) - -
Multiplex 197198 X (19) - - X (19) - X (19) - - - -
240415 - X (21) - X (21) X (21) - - - - -
197198 X (19) - - X (19) - X (19) X (19) - - -
234328 - - X (26) - X (26) X (27) - X (27) - -
  1. Adequate controls were carried, in triplicate, both in singleplex and multiplex. Multiplex reaction could not be performed with the combination of 240415 and 234328, because of limitations of the calibrated fluorophores and filter-limitations of the instrument. For each positive assay, in parenthesis is the mean ct value from triplicates with 2 ng of DNA per essay