Fig. 5
From: On the control mechanisms of the nitrite level in Escherichia coli cells: the mathematical model
Contribution of the NO2 utilization and transport system various components to the nitrite accumulation dynamics. Dots – experimental data on the nitrite utilization by the cell culture population, calculated with the kflow(s-u)/C law based on the data from [7]; curves 1-5 – (1) model calculation; curves 6-8 – (s1−7) model calculation; curve 1 – the rate of nitrite utilization by the perilasmic NrfA reductase; curve 2 (dashed) – the rate of nitrite import by the NirC transporter; curve 3 – the rate of nitrite export by the NirC transporter, measured in negative units; curve 4 – the rate of nitrite utilization by the cytoplasmic NirB reductase; curve 5 – the total rate of nitrite utilization by NrfA and NirB reductases; curve 6 – the rate of nitrite utilization by the NrfА reductase, implemented in the (s1−7) model, excluding the effect of the membrane potential (dU = 0) on the NirA and NirB proteins diffusion rate kt Nrf,cp = 0.055 s−1; curve 7 – difference between curves 1 and 6; curve 8 – the total rate of nitrite assimilation by the cell, calculated with (s1−7). Other parameters values are presented in the Appendix: Table 1