Table 4 Primers used in this study

pw30

GTATTAGAGCTCATCGCACTGCTTCTTGAGGT

For tdaA insertional mutation, forward, with SacI site

pw31

CGACTATCTAGAGATGATTGGGTCCTTTGCAC

For tdaA insertional mutation, reverse, with XbaI site

pw32

GTATTAGAGCTCAGCAGCCATGAATAGCCTGT

For tdaB insertional mutation, forward, with SacI site

pw33

CGACTATCTAGAGGGTATCGGATTTCGGATTT

For tdaB insertional mutation, reverse, with XbaI site

pw36

GTATTAGAGCTCATCTTTGGCTCCATCGACAT

For tdbD insertional mutation, forward, with SacI site

pw37

CGACTATCTAGAGCACATTGTTGGGAAACTGA

For tdbD insertional mutation, reverse, with XbaI site

pw108

GAAGAGCTCGGACGACTATGTGATTGGTCAGGC

For clpX insertional mutation, forward, with SacI site

pw109

GGGTCTAGACGACGTTATATTCCGACGCCTGCA

For clpX insertional mutation, reverse, with XbaI site

pw153

GTATTAGAGCTCGAGCATAACCGCTTTGCCCGCCGCCCA

For exoP insertional mutation, forward, with SacI site

pw154

CGACTATCTAGACCATGCTGAGTGCAAGGTTGACGGCGG

For exoP insertional mutation, reverse, with XbaI site

pw127

GCATTAGAGCTCGTCAGATTGGCCGAAGCCCCTTTT

For clpX in trans complement, forward, with SacI site

pw128

CGGCTATCTAGACGAACTCACCACCTGAGGAGATACGT

For clpX in trans complement, reverse, with XbaI site

pw166

GTATTAGAGCTCCCCGTCCGATGTGTCAAAATAGGT

For exoP in trans complement, forward, with SacI site

pw165

CGTCTTTCTAGAGGTGCCTGCGGTCATCACCATGAC

For exoP in trans complement, reverse, with XbaI site

pwGFP-F

GCGGTACATATGTAAGGAGGAAAAACATATG

For amplification of gfp ORF, forward, with NdeI site

pwGFP-R

CTATATGGATCCCAGATCTATTTGTATAGTTCATCCA

For amplification of gfp ORF, reverse, with BamHI site

Pm113

GGTACCTGTCTGTCGCCTCTTGT

For amplification of PflaB, forward, with KpnI site

Pm114

GGTACCATATCATTCCTCCATGAT

For amplification of PflaB, forward, with KpnI site

pwmO-F

GCGGTACATATGATGGTGAGCAAGGGCGAGGAGAAT

For amplification of ofp ORF, forward, with NdeI site

pwmO-R

CTATATGGATCCCTTGTACAGCTCGTCCATGCCGCC

For amplification of ofp ORF, reverse, with BamHI site