Table 3 Bacterial strains and plasmids used in this study

S4

Previously Phaeobacter sp. S4; wild type isolate from the inner shell of oysters

Karim et al., 2013

S4Sm

Spontaneous Sm^r mutant of S4

Sm^r

this study

WZ10

clpX insertional mutant of S4Sm

Sm^r Cm^r

this study

WZ11

clpX+, clpX in trans complement of WZ10

Sm^r Cm^r Ap^r

this study

WZ20

exoP insertional mutant of S4Sm

Sm^r Cm^r

this study

WZ21

exoP+, exoP in trans complement of WZ20

Sm^r Cm^r Ap^r

this study

WZ02

S4Sm (pRhokHi-2-ofp)

Sm^r Cm^r Km^r

this study

WZ12

clpX, WZ10 (pRhokHi-2-ofp)

Sm^r Cm^r Km^r

this study

WZ22

exoP, WZ20 (pRhokHi-2-ofp)

Sm^r Cm^r Km^r

this study

RE22

Wild type isolate from oyster larvae

Estes et al., 2004

RE22Sm

Spontaneous Sm^r mutant of RE22

Sm^r

this study

WZ103

RE22Sm (pRhokHi-2-gfp)

Sm^r Ap^r

this study

NB10

Wild type, serotype O1, clinical isolate from the Gulf of Bothnia

Norqvist et al., 1989

NB10Sm

Spontaneous Sm^r mutant of NB10

Sm^r

this study

WZ203

NB10Sm (pSUP202P-PflaB-gfp)

Sm^r Ap^r Tet^r

this study

CV919-312^T

Wild type isolate from a JOD-affected oyster

Boettcher et al., 1999

CV919Sm

Spontaneous Sm^r mutant of CV919-312 T

Sm^r

this study

Sm10

thi thr leu tonA lacY supE recA RP4-2 Tc::Mu::Km (λpir)

Km^r

Simon et al., 1983

S100

Sm10 harboring pNQ705-1

this study

WQ10

Sm10 harboring pNQ705-clpX

this study

WQ20

Sm10 harboring pNQ705-exoP

this study

WB01

Sm10 harboring pBBR1MCS4

this study

WB11

Sm10 harboring pBBR1MCS4-clpX

this study

WB21

Sm10 harboring pBBR1MCS4-exoP

this study

S122

Sm10 harboring pSUP202P-gfp(ORF)

this study

S136

Sm10 harboring pSUP202P-PflaB-gfp

this study

W900

Sm10 harboring pRhokHi-2-FbFP

this study

WR03

Sm10 harboring pRhokHi-2-gfp

this study

WR02

Sm10 harboring pRhokHi-2-ofp

this study

W901

Sm10 harboring pmOrange

this study

pNQ705-1

Cm^r; suicide vector with R6K origin

Mcgee, 1996

pNQ705-clpX

Cm^r; derivative from pNQ705-1 for clpX insertional mutant

this study

pNQ705-exoP

Cm^r; derivative from pNQ705-1 for exoP insertional mutant

this study

pBBR1MCS4

Ap^r; derivative from pBBR1MCS (a broad-host-range cloning vector)

Kovach et al., 1995

pBBR1MCS4-clpX

Apr; derivative from pBBR1MCS4 for clpX in trans complement

this study

pBBR1MCS4-exoP

Apr; derivative from pBBR1MCS4 for exoP in trans complement

this study

pBS(gfp)-Pcampy

Template for gfp ORF PCR amplification

Eggers et al., 2004

pCE320(gfp)-PflaB

Template for PflaB PCR amplification

Eggers et al., 2004

pSUP202P

Ap^r Cm^r Tc^r; broad host shuttle vector

Simon et al., 1983

pSUP202P-gfp(ORF)

Ap^r Tc^r; derivative from pSUP202 for GFP tagging

this study

pSUP202P-PflaB-gfp

Ap^r Tc^r; derivative from pSUP202 for GFP tagging

this study

pRhokHi-2-FbFP

Cm^r Km^r; derivative from pBBR1MCS (a broad-host-range cloning vector) with promoter PaphII

Piekarski et al., 2009

pRhokHi-2-gfp

Cm^r Km^r; derivative from pRhokHi-2-FbFP with gfp under the control of PaphII

this study

pmOrange

Template for ofp ORF PCR amplification

Clontech Laboratories, Inc.

pRhokHi-2-ofp

Cm^rKm^r; derivative from pRhokHi-2-FbFP with ofp under the control of PaphII

this study