Fig. 2

Competition assay between OFP-labeled P. inhibens S4 strains and GFP-labeled V. tubiashii. P. inhibens stains were allowed to grow and form biofilms on the glass surfaces for 24 h before the addition of V. tubiashii RE22Sm-GFP. The mixed cultures are S4Sm-OFP with RE22Sm-GFP, clpX-OFP with RE22Sm-GFP and exoP-OFP with RE22Sm-GFP. a Merged confocal microscopy images of mixed biofilm development by OFP-producing strains (S4Sm, clpX mutant and exoP mutant) and GFP-producing V. tubiashii (RE22Sm) strain on the surface of glass coverslip at 72 h. The data presented are from a representative experiment of two independent experiments. b Growth of sessile P. inhibens S4Sm strains (S4Sm, clpX, and exoP) and V. tubiashii RE22Sm in a co-culture system. c Growth of planktonic P. inhibens S4Sm strains (S4Sm, clpX, and exoP) and V. tubiashii RE22Sm in a co-culture system. The data presented are average of two independent experiments and each independent experiment has three replicates. Error bars represent one standard deviation