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Fig. 3 | BMC Microbiology

Fig. 3

From: A sensitive bacterial-growth-based test reveals how intestinal Bacteroides meet their porphyrin requirement

Fig. 3

Bt growth stimulation as a function of heme concentrations. a. The amount of heme needed to stimulate Bt growth was determined by loading 2-fold dilutions of heme ranging from 256 to 0.5 ng per well. The lowest heme concentration detectably stimulating Bt growth was between 1-4 ng. b. Area of growth stimulation was quantified using ImageJ (V1.45 s; Wayne Rasband, National Institute of Health, USA). In black, the logarithmic regression curve of the equation y = 20600ln(x) + 21091, which describes the correlation between the amount of heme and the surface area of stimulated Bt growth, with a correlation coefficient of R2 = 0.99

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