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Table 4 Product formation in MDM

From: Time-resolved amino acid uptake of Clostridium difficile 630Δerm and concomitant fermentation product and toxin formation

Product tfirst detection thalfmaximal production Maximal relative production rate
  min min h−1
Formate 30 202 ± 18 0.16 ± 0.02
2-hydroxyisocaproate 135 225 ± 12 0.21 ± 0.02
Isocaproate 90 239 ± 14 0.14 ± 0.01
Isobutanoate 135 258 ± 39 0.12 ± 0.02
Isovalerate 30 280 ± 9 0.13 ± 0.02
2-methylbutanoate 30 290 ± 43 0.11 ± 0.01
5-aminovalerate 210 360 ± 8 0.21 ± 0.02
2-oxoisocaproate 280 435 ± 16 0.15 ± 0.02
Alanine 280 442 ± 18 0.18 ± 0.01
Acetate 280 457 ± 20 0.12 ± 0.01
Ammonium traces in medium 470 ± 49 0.11 ± 0.01
  1. All compounds with the exception of formate and ammonium were detected by GC-MS; formate and ammonium were quantified by enzymatic assays (see Methods), total sampling range up to 720 min, for detailed fits, see Additional file 2