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Table 3 Exported metabolites detected in growth with CDMM

From: Time-resolved amino acid uptake of Clostridium difficile 630Δerm and concomitant fermentation product and toxin formation

Product tfirst detection thalfmaximal production Maximal relative production rate
  min min h−1
Isovalerate 60 83 ± 5 0.59 ± 0.02
Propanoate 80 120 ± 8 0.39 ± 0.01
2-oxo-isocaproate 90 155 ± 12 0.26 ± 0.08
(4-hydroxyphenyl)acetate 150 161 ± 12 0.39 ± 0.04
5-aminovalerate 60 163 ± 7 0.33 ± 0.03
2-methylbutanoate 60 168 ± 13 0.45 ± 0.05
Acetate 90 175 ± 6 0.30 ± 0.02
3-(4-hydroxyphenyl)lactate 175 181 ± 5 0.78 ± 0.14
Isocaproate 60 194 ± 7 0.47 ± 0.05
Isobutanoate 60 202 ± 5 0.59 ± 0.04
Ammonium traces in medium 210 ± 28 0.19 ± 0.04
2-aminobutanoate 150 213 ± 6 0.28 ± 0.02
Formate 60 228 ± 10 0.24 ± 0.01
Phenylacetate 60 231 ± 4 0.38 ± 0.03
Ethanol 210 259 ± 6 0.32 ± 0.04
3-hydroxybutanoate 240 261 ± 7 0.62 ± 0.13
p-cresol 60 263 ± 9 0.26 ± 0.02
2-hydroxybutanoate 150 273 ± 4 0.30 ± 0.03
1-butanol 225 274 ± 9 0.39 ± 0.03
Isobutanol 240 276 ± 8 0.46 ± 0.03
Butanoate 175 285 ± 6 0.30 ± 0.04
Valerate 175 315 ± 4 0.25 ± 0.06
3-phenylpropanoate 175 355 ± 9 0.21 ± 0.06
Lactate 225 364 ± 3 0.27 ± 0.02
3-phenyllactate 120 387 ± 21 0.12 ± 0.01
  1. All compounds with the exception of formate and ammonium were detected by GC-MS; formate and ammonium were quantified by enzymatic assays (see Methods), total sampling range up to 660 min, for detailed fits, see Additional file 3