Time-resolved amino acid uptake of Clostridium difficile 630Δerm and concomitant fermentation product and toxin formation

Table 2 Substrate uptake of amino acids and glucose supplied in MDM

Substrate	Initial concentration	Relative abundance in stationary phase	t_{halfmaximal comsumption}	Maximal relative uptake rate
	g/L	%	min	h⁻¹
Glucose	2.0	bdl^a	197 ± 10	0.17 ± 0.01
Methionine	0.2	bdl	136 ± 17	0.23 ± 0.04
Cysteine	2.0	bdl	164 ± 13	0.21 ± 0.01
Isoleucine	0.3	18.4 ± 0.8	306 ± 18	0.11 ± 0.01
Tryptophan	0.1	bdl	307 ± 23	0.13 ± 0.01
Proline	2.0	bdl	318 ± 4	0.56 ± 0.05
Leucine	0.4	27.2 ± 0.2	332 ± 8	0.16 ± 0.01
Valine	0.3	36.3 ± 0.9	347 ± 8	0.18 ± 0.02

Total sampling range up to 720 min, for detailed fits, see Additional file 2; ^abdl, below detection limit

ISSN: 1471-2180