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Table 1 Substrate uptake of amino acids and glucose supplied in CDMM

From: Time-resolved amino acid uptake of Clostridium difficile 630Δerm and concomitant fermentation product and toxin formation

Substrate Initial concentration Relative abundance in stationary phase thalfmaximal comsumption Maximal relative uptake rate
  g/L % min h−1
Glucose 2.0 bdla 246 ± 3 0.61 ± 0.07
Proline 0.36 bdl 106 ± 4 0.32 ± 0.02
Cysteine 0.5 bdl 124 ± 4 0.43 ± 0.03
Aspartate 0.27 16.3 ± 0.9 141 ± 8 0.34 ± 0.04
Arginine 0.14 35.7 ± 0.9 148 ± 7 0.20 ± 0.02
Alanine 0.16 19.4 ± 2.8 157 ± 11 0.23 ± 0.02
Leucine 0.38 bdl 161 ± 4 0.49 ± 0.08
Glycine 0.05 bdl 163 ± 7 0.31 ± 0.02
Tryptophan 0.1 45.7 ± 1.0 167 ± 15 0.12 ± 0.01
Serine 0.25 bdl 167 ± 2 0.62 ± 0.03
Isoleucine 0.24 bdl 170 ± 5 0.33 ± 0.02
Methionine 0.12 bdl 170 ± 7 0.45 ± 0.03
Tyrosine 0.24 25.7 ± 0.6 171 ± 17 0.19 ± 0.02
Threonine 0.21 bdl 177 ± 18 0.21 ± 0.01
Phenylalanine 0.20 bdl 177 ± 4 0.44 ± 0.01
Valine 0.27 5.4 ± 0.3 224 ± 13 0.24 ± 0.03
Lysine 0.34 101.0 ± 2.1 No significant consumption
Glutamate 0.80 97.6 ± 3.9 No significant consumption
  1. Composition of casamino acids is based on distribution of amino acids in casein and was confirmed by enzymatic assays (for detailed procedure see Additional file 4); total sampling range up to 660 min, for detailed fits, see Additional file 1; abdl, below detection limit