Fig. 1

Binding assay: a Binding to fibronectin of 40 μL of bacteria culture growth of BA2103, 2271-1/85 and DH5α strains incubated in ELISA microplates previously coated with cellular fibronectin, or BSA. b Serial dilutions of BA2103 incubated in ELISA microplates previously coated with cellular fibronectin (hatched bars) or BSA (white bars). c BA2103 preincubated with increasing concentrations of cellular fibronectin prior to incubation in ELISA microplates previously coated with cellular fibronectin. Adhered bacteria were recovered with 0.05 % triton-X100 in PBS and plated on LB agar in serial dilutions and then the colony-forming units were counted. Differences were statistically significant comparing the binding to fibronectin of BA2103 and the other two strains (p < 0.0001) and comparing the binding of BA2103 to fibronectin or to BSA independent of bacteria concentration (p < 0.004). It was also statistically significant compared the preincubation in absence of fibronectin or in presence of dose-dependent of fibronectin (from p < 0.03 to 0.0005). d O26:H11 strains screened by binding ability to cellular fibronectin employing the crystal violet staining measurement. Absorbance’s means between high and low binding groups were statistically significant (p < 0.0001, R² 0.9025) also means difference between high and intermediate were statistically significant (p = 0.0310)