Table 2 Primers used in this study
From: Increased toxin expression in a Clostridium difficile mfd mutant
Primer name | Sequence (5’ to 3’) | Used for |
|---|---|---|
SW78 | TCTACCACTGAAGCATTAC | qPCR for tcdA |
SW79 | TAGGTACTGTAGGTTTATTG | qPCR for tcdA |
SW80 | ACCATATAGCTTTGTGATAGTGAAGGAAA | qPCR for tcdB |
SW81 | AAGAACTACATCAGGTAATTCAGATACAAA | qPCR for tcdB |
SW82 | GGATGATATGATGAAGGTTAGAAACCT | qPCR for rpoB |
SW83 | CCCAATCCAAGTTCTTCTAGTTTTTG | qPCR for rpoB |
NF408 | TCTTGAATATCAAAGGTGAGCCAGTACA | 16S RNA amplfication |
NF409 | TACAGCGTGGACTACCAGGGTATCTAAT | 16S RNA amplfication |
NF2234 | GATCGAGCTC AATATAATGGATAGTGAGAG | mfd amplication |
NF2298 | GATCGAGCTC AATATAATGGATAGTGAGAG | mfd amplication |