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Table 1 Strains and plasmids used in this study

From: Increased toxin expression in a Clostridium difficile mfd mutant

Strain or plasmid Relevant characteristics Source or reference
C. difficile strains
630Δerm Wild-type strain Obtained from Peter Mullany [29]
630-911 (mfd mutant) 630Δerm mfd::ermB This study
630 tcdA tcdB 630Δerm tcdA::catP tcdB::erm Obtained from Nigel Minton; [43]
Plasmids
pMTL960 Plasmid vector for complementation studies Obtained from Nigel Minton; [38]
pSEW070 pMLT960 carrying wild type mfd gene under control of Pcwp2 This study