Table 1 Strains and plasmids used in this study
From: Increased toxin expression in a Clostridium difficile mfd mutant
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
C. difficile strains | ||
630Δerm | Wild-type strain | Obtained from Peter Mullany [29] |
630-911 (mfd mutant) | 630Δerm mfd::ermB | This study |
630 tcdA tcdB | 630Δerm tcdA::catP tcdB::erm | Obtained from Nigel Minton; [43] |
Plasmids | ||
pMTL960 | Plasmid vector for complementation studies | Obtained from Nigel Minton; [38] |
pSEW070 | pMLT960 carrying wild type mfd gene under control of Pcwp2 | This study |