Skip to main content
Fig. 5 | BMC Microbiology

Fig. 5

From: Increased toxin expression in a Clostridium difficile mfd mutant

Fig. 5

Toxin A and B production in the mfd mutant. Cultures of 630Δerm (1) the mfd mutant (2) and the complemented strain (3) were grown in TY or TYG media. Cell lysates and culture supernatants were prepared at various time points and analyzed by Coomassie Blue stained 6 % SDS-PAGE (a) and by Western blotting using anti-toxin A (b) or anti-toxin B antibodies (c). The addition of glucose to the TY media to form TYG is indicated. The stained bands migrating above the 212 kDa marker in panel a are presumed to be a mixture of toxin A and toxin B, as these proteins tend to co-migrate and smear on gels

Back to article page