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Fig. 2 | BMC Microbiology

Fig. 2

From: Increased toxin expression in a Clostridium difficile mfd mutant

Fig. 2

Cell wall proteins and culture supernatants of the mfd mutant. Cultures were grown in BHIS broth and cell wall proteins and culture supernatants were prepared and analyzed by 12 % SDS-PAGE. A high molecular weight band () was observed in the culture supernatant of the mfd mutant, which was identified as toxin A. The phase variable CwpV protein is observed in the parental Δerm culture (). CWP, cell wall proteins; SN, culture supernatants; M, molecular weight markers

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