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Fig. 2 | BMC Microbiology

Fig. 2

From: Increased toxin expression in a Clostridium difficile mfd mutant

Fig. 2

Cell wall proteins and culture supernatants of the mfd mutant. Cultures were grown in BHIS broth and cell wall proteins and culture supernatants were prepared and analyzed by 12 % SDS-PAGE. A high molecular weight band (▶) was observed in the culture supernatant of the mfd mutant, which was identified as toxin A. The phase variable CwpV protein is observed in the parental Δerm culture (▷). CWP, cell wall proteins; SN, culture supernatants; M, molecular weight markers

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