Table 1 PCR primers used in this study
From: Microbiological testing of pharmaceuticals and cosmetics in Egypt
Bacterial target/Primer name | Forward (F) and Reverse (R) primer Annealing temperature (tm-5 °C) | Nucleo-tide positionsa | Gene amplified | PCR product size (bp) | Ref. |
|---|---|---|---|---|---|
Universal primer pair | F: 5′AGAGTTTGATCMTGGCTCAG3′ | NP | 16S rRNA | 1500 | [27] |
R: 5′TACGGYACCTTGTTACGACTT3′ | |||||
(46.8 °C) | |||||
S. hominis | F: 5′GTTCGATAGTGAAAGATGGCTC3′ | NP | 16S rRNA | 833-852 | [24] |
R: 5′GGAAACTTCTATCTCTAGAAGG3′ | |||||
(43.4 °C) | |||||
S. warneri | F: 5′GGTTCAATAGTGAAAGGCGGC3′ | NP | 16S rRNA | 833-852 | [24] |
R: 5′GGAAGACTCTATCTCTAGAGC3′ | |||||
(41.1 °C) | |||||
S. epidermidis | F: 5′TCTCTTTTAATTTCATCTTCAATTCCATAG3′ | 448-477 | b | 174 | [25] |
R: 5′AAACACAATTACAGTCTGTTATCCATATC3′ | 593-622 | ||||
(54 °C) | |||||
B. anthracis | F: 5′AATCGTAATATTAAACTGACG3′ | 607-627 | gyrB | 244 | [26] |
R: 5′CCTTCATACGTGTGAATGTTG3′ | 831-851 | ||||
(40.5 °C) | |||||
B. cereus | F: 5′ATTGGTGACACCGATCAAACA3′ | 490-510 | gyrB | 364 | [26] |
R: 5′TCATACGTATGGATGTTATTC3′ | 834 -854 | ||||
(41 °C) | |||||
B. subtilis | F:5′CAGTCAGGAAATGCGTACGTC CTT3′ | NP | gyrA | 1027 | [26] |
R:5′CAAGGTAATGCTCCAGGCATTGCT3′ | |||||
(57.2 °C) | |||||
S. aureus | F: 5′GCGATTGATGGTGATACGGTT3′ | 48-70 | nucA (nuclease A) | 280 | [32] |
R: 5′AGCCAAGCCTTGACGAACTAA AGC3′ | 303-328 | ||||
(55 °C) | |||||
E. coli | F: 5′AAAACGGCAAGAAAAAGCAG3′ | 754-773 | uidA (β-D-glucuro-nidase) | 147 | [31] |
R: 5′ACGCGTGGTTACAGTCTTGCG3′ | 880-900 | ||||
(50.7 °C) | |||||
S. enterica | F: 5′ATCGCCACGTTCGGGCAATTC3′ | NP | invA (invasion protein) | 275 | [33] |
R: 5′ACGGTTCCTTTGACGGTGCGAT3′ | |||||
(55 °C) | |||||
P. aeruginosa | F: 5′ATGGAAATGCTGAAATTCGGC3′ | NP | oprL (membrane lipoprotein) | 504 | |
R: 5′CTTCTTCAGCTCGACGCGACG3′ | |||||
(55 °C) |