Fig. 5

PCR amplification of specific gene fragments in selected strains. PCR amplification products of the oprL gene fragment (500bp) in P. aeruginosa ATCC 9027 (Lane 1); uidA gene fragment (147 bp) in E. coli NCTC 10418 (Lane 2); nucA gene fragment (280 bp) in S. aureus ATCC 6538P (Lane 3) and invA gene fragment (275 bp) in Sal. enterica ATCC 14028 using 20 picomoles/μL and 100 picomoles/μL species-specific primer pair, respectively. Lane M shows 100 bp plus DNA ladder of fragments sizes (bp; from top to bottom): 3000, 2000, 1500, 1200, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100