Fig. 5

Real-time PCR analysis of the formation of HIV-1 DNAs. a Schematic representation of the reverse transcription and integration of HIV-1 genome. The primers used to amplify the viral DNAs were indicated. b-d. Real-time PCR analysis of HIV-1 DNA in 293 T cells. 293 T cells were infected with VSV-G pseudotyped HIV-1-GFP in the presence of DMSO (control) or SJP-L-5 at indicated concentrations. AZT (10 μM) was used as a positive control. At 24 h post infection, low-molecular-weight DNA, including the viral DNA, was isolated. The viral -sssDNA (b), flDNA (c), and 2-LTR circular DNA (d) levels were measured by real-time PCR. Error bars indicate standard deviations of triplicate values. Statistical significance was analyzed by the Student’s t test. ^* P < 0.05‚ ^** P < 0.01 and ^*** P < 0.001 versus the negative control