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Table 2 Quantitation of hemolytic activity of the recombinant wild type and mutant BPSL1375 proteins

From: Characterization of Burkholderia pseudomallei protein BPSL1375 validates the Putative hemolytic activity of the COG3176 N-Acyltransferase family

Protein / Control

Hemolysis Activity (%)a

Distilled water

100

B. pseudomallei D286 lysate

80

Wild type recombinant BPSL1375

27

Recombinant R54K

14

Recombinant D75E

18

Recombinant D78E

61

Recombinant R99K

7

Recombinant E132D

22

Recombinant R135K

5

PBS

9

  1. aIncubation with erythrocytes for 60 min at 37 °C
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BMC Microbiology

ISSN: 1471-2180

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