Table 1 Primers used for PCR amplification of bpsl1375 and site directed mutagenesis of bpsl1375
Primer name | Primer Sequence (5’-3’) |
|---|---|
BPSL1375_F | CACCATGCGAGAACTGCCGA |
BPSL1375_R | GCGCGGCAGCGGATCGCTCA |
BPSL1375_R54K_F | GAAGCGCAGCGGCTGAAGCACAGTGTGTTCGCC |
BPSL1375_R54K_R | GGCGAACACACTGTGCTTCAGCCGCTGCGCTTC |
BPSL1375_D75E_F | GGCCTCGACGTCGAGCCGTTCGACC |
BPSL1375_D75E_R | GGTCGAACGGCTCGACGTCGAGGCC |
BPSL1375_D78E_F | GACGTCGATCCGTTCGAGCCGTACTGC |
BPSL1375_D78E_R | GCAGTACGGCTCGAACGGATCGACGTC |
BPSL1375_R99K_F | TGAAGGTCGTCGGCACCTATAAGGTGCTGCCGCC |
BPSL1375_R99K_R | GGCGGCAGCACCTTATAGGTGCCGACGACCTTCA |
BPSL1375_E132D_F | CGAAGATGGTCGATGTCGGCCGCTCGT |
BPSL1375_E132D_R | ACGAGCGGCCGACATCGACCATCTTCG |
BPSL1375_R135K_F | GATGGTCGAAGTCGGCAAGTCGTGCGTGCATCGCG |
BPSL1375_R135K_R | CGCGATGCACGCACGACTTGCCGACTTCGACCATC |