Fig. 3

A comparison between the electron micrographs of cytoplasmic blebbing on the membrane and recombinant E. coli carrying the bpsl1375 gene grown on blood agar plates. Scanning electron microscopy revealed that sheep erythrocytes treated with the recombinant BPSL1375 displayed crenated shapes with cytoplasmic blebbing on the membrane (a) compared to untreated erythrocytes that presented the usual biconcave discs appearance (b), nevertheless, blebbing was not as notable as the positive control for lysis (c). Recombinant E. coli carrying the bpsl1375 gene (see below) that were grown on blood agar plates also showed clear hemolytic zones (d). a Normal biconcave disc shape of untreated erythrocytes in PBS; b BPSL1375 treated erythrocytes showing the presence of discrete blebs (yellow arrow) on the erythrocyte; and c erythrocytes in sterile distilled water that also show the blebs (yellow arrow) as a positive control for lysis. d Recombinant E. coli bpsl1375colonies grown on trypticase soy agar with 5 % sheep blood after 24, 48 and 72 h of incubation at 37 °C showing clear hemolytic zones