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Fig. 5 | BMC Microbiology

Fig. 5

From: Identification of a Golgi-localized UDP-N-acetylglucosamine transporter in Trypanosoma cruzi

Fig. 5

TcNST1 is expressed at the mRNA level in all life forms of T. cruzi and during metacyclogenesis in vitro. RT-PCR analysis of TcNST1 mRNA expression in epimastigotes (Epi), cell-culture-derived trypomastigotes (Trypo), amastigotes (Ama), metacyclics (Meta) and intermediary forms during metacyclogenesis: epimastigotes in the late exponential phase (LEP), epimastigotes under nutritional stress (Stress) and adhered epimastigotes (Adh). Expression of the 60S ribosomal protein L9 was used as an internal control (TcCLB.504181.10). Aliquots of all samples were pooled together and subjected to reverse transcription without reverse transcriptase (− RT)

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