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Fig. 1 | BMC Microbiology

Fig. 1

From: Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

Fig. 1

NTHi penetration of bronchial epithelial cells. BEAS-2B cells were infected with one of two NTHi strains (ATCC 19418 or HUSM 0481), E. coli (a negative control), or L. monocytogenes (a positive control) for 2 hours. After killing extracellular bacteria with gentamicin, epithelial cells and bacteria were stained with LIVE/DEAD® and Hoechst. a Representative fluorescence micrographs of L. monocytogenes, and NTHi strain ATCC 19418. White arrows show viable intracellular bacteria stained green. White bars represent 5 μm. Fluorescent micrographs were taken at 2,000× magnification. b The percentages of epithelial cells invaded by bacteria. c After killing extracellular bacteria with gentamicin, cells were lysed and the bacteria were cultured overnight. The number of colonies was counted and the percentages of CFU after gentamicin treatment per input CFU were shown. Error bars represent SEM in three independent experiments that gave similar results. *p < 0.05 and **p < 0.01

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BMC Microbiology

ISSN: 1471-2180

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