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Table 3 Summary of tyrosine decarboxylase BLAST analysis

From: High-throughput DNA sequencing to survey bacterial histidine and tyrosine decarboxylases in raw milk cheeses

Cheese

# of Clones

Gene target

BLAST output

E-value

% Identity

Irish Artisanal Cheese A

5

tdc

E. faecalis tdc operon complete cds

0

99 %

 

1

tdc

E. faecalis tdc operon complete cds

1.0E-141

99 %

 

2

tdc

Lb. curvatus tdc complete cds/S. thermophilus tdcA gene

0

99 %

Reblochon

5

tdc

Lb. curvatus tdc /S. thermophilus tdcA gene complete cds

0

99 %

 

1

tdc

E. faecalis tdc gene, complete cds

0

100 %

Irish Artisanal Cheese B

8

tdc

Lb. curvatus tdc, complete cds/S. thermophilus tdcA gene

0

99 %

Tête de Moine

7

tdc

E. faecalis tdc gene, complete cds

0

98 %

 

1

tdc

Lb. plantarum/Lb. brevis tdc gene cds

0

99 %

Pecorino Sardo

6

tdc

E. faecium tyrS gene, tyrdc gene complete cds

0

99 %

 

1

tdc

E. faecium tyrS gene, tyrdc gene complete cds

2E-70

79 %

 

1

tdc

E. faecium tyrS gene, tyrdc gene complete cds

0

89 %

Ossau-Iraty

2

tdc

E. faecalis, tdc gene complete cds

0

98 %

 

2

tdc

E. faecalis complete genome

0

97 %

 

1

tdc

E. faecalis complete genome

0

99 %

 

1

tdc

Lb. curvatus tdc gene complete cds/S. thermophilus tdcA gene

0

99 %

  1. Summary of homologues of tyrosine decarboxylase (tdc) gene fragments detected in tdc positive cheeses using Sanger sequencing of cloned amplicons