Table 2 Comparative evaluation of DENV serotype-specific RT-LAMP assay with RT-PCR, and real-time PCR for detection of DENV stains and the patients’ serum samples
Type of case | Virus serotype | No. of samples Virus isolation | No. of samples positive by: | ||
|---|---|---|---|---|---|
RT-LAMP | RT-PCR | Real-time PCR | |||
Virus stains | DEN-1 | 20 | 20 | 18 | 20 |
DEN-2 | 30 | 30 | 27 | 30 | |
DEN-3 | 15 | 15 | 15 | 15 | |
DEN-4 | 15 | 15 | 14 | 15 | |
Total | 80 | 80 | 74 | 80 | |
Accuracy (%) | 100 | 92.5 | 100 | ||
Patients’ serum samples | DEN-1 | 50 | 50 | 41 | 44 |
DEN-2 | 60 | 59 | 52 | 54 | |
DEN-3 | 40 | 40 | 35 | 38 | |
DEN-4 | 40 | 39 | 33 | 36 | |
Total | 190 | 188 | 161 | 172 | |
Accuracy (%) | 98.95 | 84.74 | 90.53 | ||
Healthy | 20 | 0 | 0 | 0 | |
Total | 20 | 0 | 0 | 0 | |
False positive rate (%) | 0 | 0 | 0 | ||