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Table 1 Bacterial strains used in this investigation and macrophage phenotypes following infection

From: Characterization of the murine macrophage response to infection with virulent and avirulent Burkholderia species

Species and strain Source Location Year isolated Genome sequenced Actin tails MNGC Uptake Intracellular replication In vivo Pathogenicity
B. thailandensis
aDW503 Environment Thailand 1998 Yes Yes [44, 66] Yes [44] Yes [44] Yes [44]  
 CDC3015869 Human blood Texas 2003 Yes Yes [44] Yes [44] Yes [44] Yes [44] Virulent [12]
 Phuket 4 W-1 Water Thailand Unknown Partial Yes [44] Yes [44] Yes [44] Yes [44] Virulent [12]
B. oklahomensis
 E0147 Human conjunctiva Georgia, US 1977 Yes No [44] No [44] Yes [44] Yes [44] Avirulent [12]
B. mallei
 NCTC 10229 Unknown Hungary 1961 Yes Yes Yes Yes Yes Virulent [41]
 NCTC 10247 Unknown Turkey 1960 Yes No Reduced Yes Yes Attenuated [41]
 NCTC 3708 Mule India 1932 No Yes Yes Yes Yes Virulent [41]
 NCTC 3709 Horse India 1932 Yes Yes Reduced Yes Yes Virulent [41]
 ATCC 23344 Human China 1942 Yes Yes [67] Yes [68] Yes [68] Yes Virulent [41]
b23344 ΔwcbB UGA China 1942 Yes cNT Reduced Yes Reduced  
b23344 ΔwbiL UGA China 1942 Yes cNT Yes Yes Reduced  
b23344 ΔwbiLwcbB UGA China 1942 Yes cNT Reduced Yes Reduced  
B. pseudomallei
 576 Human Blood Thailand Unknown Yes Yes [44] Reduced Yes [44] Yes [44]  
 MSHR305 Human Brain Australia 1994 Yes Yes Yes Yes Yes Virulent [43]
 295 Soil Australia Unknown No Yes Reduced Reduced Reduced  
 713 Ulcer Australia Unknown No Yes Reduced Yes Yes  
 1026b Blood Thailand 1993 Yes Yes [69] Reduced Yes [69] Reduced Virulent [43]
 DD503 Soil Australia Unknown No Yes Reduced Reduced Reduced  
 776 Blood Australia Unknown No Yes No Reduced Reduced  
 E8 Soil Thailand 1990 Yes Yes [21] Yes Yes [21] Yes [21]  
 K96243 Human Thailand 1996 Yes Yes [44] Yes [48] Yes [44] Yes [44] Virulent [42]
  1. Table 1 Bacterial strains used in this investigation and RAW264.7 macrophage phenotypes following infection. Explanations of column headings are: Species and strain: the species and strain that are used in this study; Source, location and year isolated: the source, location, and year that the corresponding strain was first identified; Genome Sequenced: the availability of the gene sequencing information for the corresponding strain; Actin tails, the ability of the corresponding strain to polymerize host actin and exhibit actin tails on the bacterial surface; MNGC, ability of the corresponding strain to induce macrophage MNGC; uptake and intracellular replication: ability of the corresponding strain to be taken up by macrophages and replicate intracellularly; in vivo pathogenicity, pathogenicity is determined by survival of mice and Syrian hamsters challenged with indicated Burkholderia spp.
  2. aDerived from B. thailandensis E264; Δ(amrR-oprA) (Kms Gms Sms); rpsL (Smr) [10]
  3. bObtained from the laboratory of Mark Schell, University of Georgia nUGA) and derived from B. mallei ATCC 23344
  4. cNT, not tested in the study