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Table 1 Strains and plasmids used in this study

From: The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917

Strain or plasmid Genotype or description Source or reference
E. coli strains   
XL1Blue recA1 lac endA1 gyrA96 thi hsdR17 supE44 relA1 (F’ proAB lacI q lacZ ΔM15 Tn10) Stratagene
DH5α F Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk mk ) phoA supE44 λ thi- gyrA96 relA1 Gibco BRL
HB101 F mcrB mrr hsdS20(rB mB ) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ ATCC 33694
S17(λpir) Tpr Smr recA thi pro hsdR hsdM +RP4::2-Tc::Mu::Km Tn7 λ Biomedal
Nissle 1917 (EcN) Non-pathogenic probiotic isolate (O6:K5:H1) Ardeypharm
EcNsat::cat Nissle 1917 sat::cat; Cmr This study
E2348/69 Wild type EPEC O127:H6 Smr B.B. Finlay
ECOR collection strains Human stool isolates [43]
Plasmids   
pBR322 Vector for cloning, Apr Tcr Biolabs
pSat Gene sat from EcN cloned in pBR322, Apr This study
pSat-S256I Gene sat from EcN encoding the mutated Sat-S256I cloned in pBR322, Apr This study
pCAT19 Source of cat gene, Apr Cmr [26]
pUT mini-Tn5 Tc tnp* gene of Tn5-IS50R inserted in SalI site of pGP704; mini-Tn5 Tc transposable element, Apr Tcr Biomedal
pFU34 Plasmid for transcriptional fusions to the reporter gene gfp mut3.1 , Apr [28]
pFU34-sat Promoter fusion Φ(sat-gfp mut3.1 ) in PFU34, Apr This study